This test measures the level of Vitamin B12 (Cyanocobalamin) in a single blood sample. Book the Vitamin B12 test at our lab in Pune or get free home sample pickup at locations near you.
The test is also called the Cyanocobalamin Test or Cobalamin Test.
Check the sample report PDF for more details. The cost of the Vitamin B12 test in Pune is Rs.800.
A vitamin B12 test is a blood test that measures the level of vitamin B12 (cyanocobalamin) in your body. This vitamin is essential for nerve function and the formation of healthy red blood cells.
It is commonly advised for people with symptoms of fatigue, weakness or nerve problems, and for those following a strict vegetarian diet who may be at risk of deficiency.

The vitamin B12 test is recommended for the following groups of people:
Your doctor may advise a Vitamin B12 test when you have symptoms that suggest a deficiency. Common signs include:
The Vitamin B12 test is a simple blood test and needs no special preparation. It is quick and nearly painless.
To prepare for the test:
To give the sample:
The vitamin B12 test report includes the following parameter:
| Sample Type | Blood |
| Time to Get Report | 12.00 Noon 1 day |
| Test Method | CLIA / CMIA |
| Normal Range | Vitamin B12 (Cyanocobalamin) : 187.0 - 883.0 pg/ml |
| Purpose of test | Checks Vitamin B12 levels to assess deficiency, anaemia and nerve health |
| Intended for | Adults with suspected Vitamin B12 deficiency, fatigue or neurological symptoms |
| Fasting Required | No |
| Total Tests | 1 |
| Doctor's Prescription Required | No |
| Reports Available By | PDF available through WhatsApp, Email and Hardcopy (on request) |
| Other Names | Vitamin B12 Test, Cyanocobalamin Test, Cobalamin Test |
The Vitamin B12 test measures the level of vitamin B12 in your blood. The table below lists the parameter along with its normal range.
| Group | Normal Range |
|---|---|
| Female | 187 – 883 |
| Male | 187 – 883 |
| Result | Meaning | Common Causes |
|---|---|---|
| Low Vitamin B12 | Deficiency (too little B12 in the blood, which can affect nerves and blood cell production) | Pernicious anaemia (an immune condition where the body cannot absorb B12 from food); strict vegetarian or vegan diet (little or no animal-based foods that carry B12); malabsorption (poor uptake of nutrients in the gut, as seen in coeliac or Crohn's disease) |
| Borderline low Vitamin B12 | Marginal status (levels near the lower limit that may cause early symptoms like tiredness or tingling) | Long-term metformin use (a diabetes medicine that reduces B12 absorption); ageing gut (older stomachs release less acid needed to free B12 from food); low dietary intake (not eating enough meat, eggs, or dairy) |
| High Vitamin B12 | Elevated levels (more B12 in the blood than expected, sometimes signalling an underlying problem) | Over-supplementation (taking high-dose B12 tablets or injections); liver disease (a damaged liver releasing stored B12 into the blood); certain blood disorders (bone marrow conditions that raise B12-carrying proteins) |
At Pathofast Lab, Pune, the Vitamin B12 test costs Rs.800. Prices are approximate and vary by lab, so confirm before booking.
No, fasting is generally not required for a standalone Vitamin B12 test. You can eat and drink normally, unless your doctor advises otherwise for a broader package.
At Pathofast, the normal Vitamin B12 range is 187.0 to 883.0 pg/ml. Ranges may vary slightly between labs, so read your report against its stated range.
Common symptoms include fatigue, weakness, numbness or tingling, pale skin and a sore tongue. Long-term deficiency can cause anaemia and nerve problems.
Vitamin B12 comes mainly from meat, fish, eggs and dairy. Many Indian vegetarians and vegans have low levels, so testing helps decide if supplements are needed.
It is a simple blood test where a small sample is drawn from a vein in your arm. You can return to normal activities immediately afterward.
At Pathofast Lab, Pune, your Vitamin B12 report is typically ready by 12:00 noon the next day. Turnaround may vary during busy periods.
Low levels may indicate deficiency or pernicious anaemia, often causing fatigue and nerve issues. Your doctor may suggest diet changes, supplements or further tests.
High B12 is usually harmless but can sometimes point to liver disease, diabetes or certain blood disorders. Discuss unexpected results with your doctor.
Consider testing if you are vegetarian or vegan, pregnant, elderly, or have symptoms like tiredness, numbness or unexplained anaemia, as advised by your doctor.
Yes, certain medicines and B12 supplements can change results. Tell your doctor about any medications or supplements, but do not stop them without advice.
In cities like Mumbai, Delhi, Bengaluru, Hyderabad and Chennai, prices are roughly Rs.400 to Rs.1300. Prices are approximate and vary by lab and location.
Pathofast Lab in Pune, is the best lab in Pune for accurate and reliable blood tests. Our lab is located conveniently in the center of Pune and we also offer home sample collection for the Vitamin B12 Test in various areas of Pune.
You can be assured of quality service and timely and accurate reports